![]() ![]() Genomic DNA is extracted from plant leaves and subjected for treatment to remove contaminant RNA, protein and polysaccharide, Genomic DNA is measured by Nanodrop (a spectrophotometer technology, not by fluorometer/Qubit) and provided in 1.5-ml tubes at a concentration of 0.5mg/ml or 1mg/ml in TE buffer. Plant Genomic DNA Zyagen offers a pure and intact high molecular size control genomic DNA of 32 different plant species.Applications: PCR Amplification Southern blot analysi Genomic DNA methylation Single-nucleotide polymorphism (SNP) Analysis Profiling studies in gene expression DNA library construction Quality Control: The integrity and purity of genomic DNA was tested by agarose gel electrophoresis and spectrophotometer Ratio of A260/280 is >1.8 Free of RNA contamination Successful PCR amplification of Beta-Actin Successful digestion of DNA by EcoR1 and/or Hind III enzyme. ![]() Genomic DNA samples of multiple donors of many species are also available upon request. Genomic DNA is measured by Nanodrop (a spectrophotometer technology, not by fluoreometer/Qubit) and provided in 1.5-ml tubes at a concentration of 0.5mg/ml or 1mg/ml in TE buffer. Genomic DNA is extracted from whole blood or tissues of single donor and treated with DNase-free RNase to remove contaminant RNA. Control Genomic DNA Zyagen offers a pure and intact high molecular size control genomic DNA of more than 40 different species (male and female).The integrity of protein is tested by immunoblotting using specific beta-actin antibody. The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein bands are verified by denatured SDS-PAGE with Coomassie blue staining. Samples are treated with DNase-free RNase to remove contaminant RNA and provided in 1.5-ml tubes at a concentration of 1mg/ml in TE buffer. DNA samples are suitable for PCR, Southern blot analysis, and DNA library construction. It is provided in RNase-free water, 1mM sodium citrate, or 0.1 mM EDTA at a concentration of 1 mg/ml. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). The integrity of each RNA sample as indicated by intact 28S and 18S ribosomal RNA is verified by denatured agarose gel electrophoresis. All RNA samples are treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR analysis, rapid amplification of cDNA ends (RACE) and purification of mRNA for library construction. Match Set of Tissue RNA, DNA, & Protein Products Zyagen offers high quality intact total RNA, DNA, and total protein simultaneously isolated of the same tissue of C57BL/6 mouse or Sprague Dawley rat. ![]()
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